Human Anti-TPO Recombinant Antibody (clone B8)

Customer Review

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Laura Scott
18.Oct,23

Great for TPO Studies

The Anti-TPO Recombinant Antibody (clone B8) has been excellent for our TPO studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

David Young
05.Dec,22

Reliable in Multiple Assays

We incorporated this antibody into our Western blot and immunoassays, and it has been a great asset. The specificity for TPO is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

Megan Martinez
09.Jul,21

Consistent High-Quality Results

This antibody has proven to be consistent and high-quality in our various assays. The reproducible results and strong affinity for TPO have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.

Q&As

1. Is the anti-TPO recombinant antibody clone B8 suitable for use in Western blotting?

   A: Yes, the anti-TPO recombinant antibody clone B8 (FAMAB-0014JF) is suitable for use in Western blotting. It provides specific binding to TPO, allowing for reliable detection in Western blot assays.

2. What are the recommended storage conditions for the anti-TPO recombinant antibody clone B8?

   A: The recommended storage condition for the anti-TPO recombinant antibody clone B8 (FAMAB-0014JF) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the anti-TPO recombinant antibody clone B8 be used in immunoprecipitation assays?

   A: Yes, the anti-TPO recombinant antibody clone B8 (FAMAB-0014JF) can be used in immunoprecipitation assays. It provides specific binding to TPO, enabling the successful precipitation of the target protein from complex mixtures.

4. Is the anti-TPO recombinant antibody clone B8 effective in ELISA applications?

   A: Yes, the anti-TPO recombinant antibody clone B8 (FAMAB-0014JF) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of TPO.

5. What is the optimal dilution for using the anti-TPO recombinant antibody clone B8 in immunofluorescence?

   A: The optimal dilution for using the anti-TPO recombinant antibody clone B8 (FAMAB-0014JF) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Gravina, Alessia, et al. "Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression." Nature biotechnology 41.5 (2023): 717-727. https://doi.org/s41587-022-01540-7
   The study investigates the protection of cell therapeutics from antibody-mediated killing through the overexpression of CD64. This approach enhances the survival and efficacy of allogeneic cell therapeutics used in cancer therapy and regenerative medicine. The research demonstrates that engineered cells overexpressing CD64 can effectively evade antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), which are primary mechanisms of antibody-mediated rejection.
   At Creative Biolabs, we provided several essential reagents for this research. These included the humanized anti-MICA IgG1 antibody (Cat#: TAB-0799CL), the humanized anti-CD52 IgG1 antibody (Cat#: FAMAB-0089WJ), the humanized anti-CD52 IgG1 antibody (Cat#: FAMAB-0014JF), and the hypusine antibody (Cat#: HPAB-0440-YJ). Our products were crucial for the experiments involving antibody-mediated killing assays, enabling the detailed examination of how engineered cells can evade immune detection and destruction. This study highlights the potential of CD64 overexpression to enhance the viability of cell-based therapies, underscoring our commitment to supporting advanced research and innovative therapeutic solutions.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# FAMAB-0014JF, RRID: AB_3111302)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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