Human Anti-TPO Recombinant Antibody (clone B8) (CAT#: FAMAB-0014JF)
Recombinant monoclonal antibody specially binds to VI of human TPO. This human anti-TPO monoclonal antibody B8 can be widely used in the treatment of Graves' disease.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in ELISA
ELISA analysis of FAMAB-0014JF was performed by coating with human TPO protein (His tag). Then blocked with BSA and incubated with FAMAB-0014JF. A HRP-conjugated goat anti-human IgG (H+L) was used as the secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in WB
Western blot analysis of FAMAB-0014JF was performed by loading human TPO protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with FAMAB-0014JF and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 4000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in DB
Dot Blot analysis of FAMAB-0014JF was performed by coating with human TPO protein (His tag).
FAMAB-0014JF incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 4000)
Specifications
- Host Species
- Human
- Derivation
- Human phage display scFv library
- Type
- Human IgG
- Specificity
- Human TPO
- Species Reactivity
- Human
- Clone
- B8
- Applications
- WB, ELISA, IHC
- Related Disease
- Graves' disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The TPO antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
Target
Related Resources
Autoimmune Thyroid Disease
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-999-F(E) | Recombinant Anti-TPO Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
| MHH-999-F(E) | Recombinant Human Anti-TPO Antibody Fab Fragment | IP, IF, FuncS | Fab |
| PFBW-123 | Human Anti-TPO Recombinant Antibody (clone TR1.9); Fab Fragment | WB, ELISA | Human Fab |
| HPAB-2017-FY-F(E) | Human Anti-TPO Recombinant Antibody (clone FY-019); Fab Fragment | ELISA | Human Fab |
| FAMAB-0364JF-F(E) | Human Anti-TPO Recombinant Antibody (clone RI-34); Fab Fragment | ELISA, FuncS | Human Fab |
Customer Reviews and Q&As
Customer Review
Q&As
Laura Scott
Great for TPO Studies
Great for TPO Studies
The Anti-TPO Recombinant Antibody (clone B8) has been excellent for our TPO studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
18.Oct,23
David Young
Reliable in Multiple Assays
Reliable in Multiple Assays
We incorporated this antibody into our Western blot and immunoassays, and it has been a great asset. The specificity for TPO is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
05.Dec,22
Megan Martinez
Consistent High-Quality Results
Consistent High-Quality Results
This antibody has proven to be consistent and high-quality in our various assays. The reproducible results and strong affinity for TPO have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.
09.Jul,21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# FAMAB-0014JF, RRID: AB_3111302)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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